Localized Capture of Bacteria in an Interdigitated Electrode Impedance Biosensor

Authors

Keywords:

biosensor, impedance spectroscopy, microchannel, interdigitated microelectrodes

Abstract

This work presents the design of a biosensor for detection of Escherichia coli bacteria. The biosensor consists of an interdigitated electrodes array to detect the impedance changes, as a function of frequency, when bacteria is captured in different areas. The design was first simulated with COMSOL Multiphysics® software to obtain the impedance magnitude and phase curves, after that the transfer function for every case was established with an algorithm in MATLAB® software to propose an equivalent circuit in order to determine the electrical components that dominate the impedance response in every frequency range of the curve. Results show that impedance changes in the biosensor are mainly due to the bacteria captured between interdigitated electrodes, whereas the bacteria captured only over interdigitated electrodes produce a very small change in the impedance. In order to confine the bacteria between electrodes, a microchannel is added, increasing the biosensor sensitivity.

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Author Biographies

Alvaro Zazueta-Gambino, Instituto Nacional de Astrofísica, Óptica y Electrónica

Alvaro Zazueta-Gambino recibió el grado de Maestro en ciencias en Electrónica por parte del Instituto Tecnológico de Mexicali, Baja California, México en 2011. Actualmente se encuentra cursando un Doctorado en Electrónica en el Instituto Nacional de Astrofísica, Óptica y Electrónica (INAOE). Su trabajo de tesis se enfoca en la fabricación de un microsensor para la detección de bacterias.

Claudia Reyes-Betanzo, Instituto Nacional de Astrofísica, Óptica y Electrónica

Claudia Reyes-Betanzo. Obtuvo su grado de Maestría en Electrónica en la Universidad Autónoma de Puebla, México en 1997, y el Doctorado en la Universidad Estatal de Campinas, Brasil en 2003. Ella es actualmente investigadora en el Instituto Nacional de Astrofísica, Óptica y Electrónica (INAOE) en Puebla, México. Sus áreas de interés incluyen biosensores para detección de bacterias, diseño y fabricación de microbombas y sistemas microfluídicos.

Víctor Manuel Jiménez-Fernández, Universidad Veracruzana

Víctor Manuel Jimenez-Fernandez recibió el grado de Doctor en ciencias en Electrónica por parte del Instituto Nacional de Astrofísica, Óptica y Electrónica (INAOE) en Puebla, México en 2006. De 2006 a 2007 fue investigador invitado en el Departamento de Ingeniería Eléctrica y de Computadoras de la Universidad Nacional del Suren Bahía Blanca, Argentina. Desde febrero de 2009 se ha desempeñado como investigador en la Universidad Veracruzana, en Veracruz, México. Sus líneas de investigación incluyen Análisis y Diseño de Circuitos Asistido por Computadora, Modelado de Sistemas No Lineales y Computación Científica.

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Published

2021-07-27

How to Cite

Zazueta-Gambino, A., Reyes-Betanzo, C., & Jiménez-Fernández, V. M. (2021). Localized Capture of Bacteria in an Interdigitated Electrode Impedance Biosensor. IEEE Latin America Transactions, 100(XXX). Retrieved from https://latamt.ieeer9.org/index.php/transactions/article/view/5424